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Lab Protocols

Lab Protocols

 

The below are somewhat random, and are added upon request. Let us know if you'd like to see a specific protocol posted.

General Cell Biology
NIH3T3 transfection with Jetprime Polyplus
Culturing Immortalized Dicer Floxed MEFs
Rubidium Chloride Competent Cell Protocol
Staining for beta-galactosidase activity
Lac Z staining mouse embryos
Freezing ES cells
Thawing ES cells
Growing/splitting E14 ES cells
Electroporation of targeting construct
Picking ES cell colonies and transferring them to 96-well plates
Passaging/Splitting 96-well Master Plate into Three Plates
Freezing 96-well master plates
DNA Isolation from ES Cells -96 well plate
24-well Plate Spin Infection
General Molecular Biology
Actin Cre genotyping
LacZ Genotyping
8-cell Embryo Collection
P32 Probe Preparation
MicroRNA and siRNA Cloning Protocol
Making Long dsRNA from In Vitro Transcriptions
A PCR-based strategy for cloning short hairpin sequences: “PCR shagging"
CIP
Cloning via Restriction Digest
Site-Directed Mutagenesis (Stratagene protocol)
Chemical Synthesis of ImpA (adenosine 5'-phosphorimidazolide)
Metabolic Labeling Using 32P
Preparing 96-well Genotyping Plate for genomic DNA Extraction
Virus
CLONING SMALL HAIRPINS INTO LENTIVIRAL VECTORS
Lentiviral Transfection Protocol for a 10cm dish
Lentiviral Harvest and Concentration
LentiLox Vector Design
Lentiviral Vectors Perivitelline Injection
Lentivirus harvest and concentration
Lentiviral Transfection
RNAi
Lipid siRNA transfection
PCR template method for dsRNA Synthesis
Lymphocyte siRNA electroporation transfection
Library cloning protocol - shRNA context
siRNA transfection with HiPerfect (MIN6 cells)
microRNA
miRNA microarrays
Small RNA Northern Blotting
Library cloning protocol - mir30 context