Dr. McManus completed his postdoctoral training as a Cancer Research Institute fellow at the intersection of RNA-based technologies and its applications to human disease. In the laboratory of Nobel Laureate Phillip A. Sharp in the Center for Cancer Research at the MIT, he studied the role of RNA-interference pathways in mammals, developing tools to probe gene regulation. Since 2004 he has been a principle investigator at UCSF, overseeing a very productive and interactive lab at UCSF, studying the basic biological processes relating to gene regulation and cell biology, using cultured cells and the mouse as a model. In 2010 Dr. McManus was awarded tenure in the Department of Microbiology and Immunology, and thereafter bestowed a Vincent and Stella Coates Endowed Chair for research excellence. He founded the W.M. Keck Center for Noncoding RNAs and a heavily used ViraCore at UCSF. At UCSF he has been granted a number of noteworthy research awards for excellence in technologies and collaboration: the Sandler Integrative Science Award, the New Technologies Award, the PBBR Science Award, Breakthrough Technologies Award, and the WM Keck Award. He was recently awarded a Transformative Award from the NIH Directors Office for his pioneering research on the reconstruction of ancestral cells by enzymatic recording. Indeed his work has a strong technology component, having developed cutting-edge research tools and large-scale resources that relate to small RNAs (microRNA, RNAi, and CRISPR) for the interrogation of gene function and their potential use in the intervention of human disease. His lab has built many mouse and human genome-scale lentiviral libraries, mouse and human genome shRNA and Cas9 libraries, pioneered novel high-throughput cell-based screening technologies, and is highly collaborative. He maintains a very diverse program, with students and postdocs and research technicians working in a variety of research areas.

recent publications

Boettcher M, Covarrubias S, Biton A, Blau J, Wang H, Zaitlen N, et al. Tracing cellular heterogeneity in pooled genetic screens via multi-level barcoding.. Vol 20.; 2019. (BMC Genomics; vol 20; no 1).
Gagnon JD, Kageyama R, Shehata HM, Fassett MS, Mar DJ, Wigton EJ, et al. miR-15/16 Restrain Memory T Cell Differentiation, Cell Cycle, and Survival.. Vol 28.; 2019. (Cell Rep; vol 28; no 8).
Yoneshiro T, Wang Q, Tajima K, Matsushita M, Maki H, Igarashi K, et al. BCAA catabolism in brown fat controls energy homeostasis through SLC25A44.. Vol 572.; 2019. (Nature; vol 572; no 7771).
Wang TA, Teo CF, Åkerblom M, Chen C, La Fontaine MT, Greiner VJ, et al. Thermoregulation via Temperature-Dependent PGD Production in Mouse Preoptic Area..; 2019. (Neuron).
Mues M, Karra L, Romero-Moya D, Wandler A, Hangauer MJ, Ksionda O, et al. High-Complexity shRNA Libraries and PI3 Kinase Inhibition in Cancer: High-Fidelity Synthetic Lethality Predictions.. Vol 27.; 2019. (Cell Rep; vol 27; no 2).
Das S, K Ansel M, Bitzer M, Breakefield XO, Charest A, Galas DJ, et al. The Extracellular RNA Communication Consortium: Establishing Foundational Knowledge and Technologies for Extracellular RNA Research.. Vol 177.; 2019. (Cell; vol 177; no 2).
Lindtner S, Catta-Preta R, Tian H, Su-Feher L, Price JD, Dickel DE, et al. Genomic Resolution of DLX-Orchestrated Transcriptional Circuits Driving Development of Forebrain GABAergic Neurons.. Vol 28.; 2019. (Cell Rep; vol 28; no 8).
Shang W, Jiang Y, Boettcher M, Ding K, Mollenauer M, Liu Z, et al. Genome-wide CRISPR screen identifies FAM49B as a key regulator of actin dynamics and T cell activation..; 2018. (Proc Natl Acad Sci USA).
Oprea TI, Jan L, Johnson GL, Roth BL, Ma'ayan A, Schürer S, et al. Far away from the lamppost.. Vol 16.; 2018. (PLoS Biol; vol 16; no 12).
Bulut-Karslioglu A, Macrae TA, Oses-Prieto JA, Covarrubias S, Percharde M, Ku G, et al. The Transcriptionally Permissive Chromatin State of Embryonic Stem Cells Is Acutely Tuned to Translational Output.. Vol 22.; 2018. (Cell Stem Cell; vol 22; no 3).