Preparing 96-well Genotyping Plate for genomic DNA Extraction

Posted by cpark | Created: 19 Feb 2009 | Last Modified: 19 Feb 2009
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Materials: 

 E14 media without LIF

Methods: 

ES cells for DNA extraction do not need to maintain pluripotency. Use media that does not contain LIF.

Grow cells over-confluent. Over-confluent plates will yield higher DNA concentration.

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  1. Grow cells 2-3 days longer than the freezing plates. 
  2. Change media everyday or twice a day until the cells are ready for DNA extraction.
  3. Once the plate is ready, aspirate media and rinse the cells with 150ul of PBS.
  4. The plate is ready to genotype, or can be stored at -20˚C.

 

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