yusuke.totoki-ohnishi



My projects relate to the development of novel CRISPR screening platforms. I completed my postdoctoral training at the EMBL Heidelberg where I focused on the mechanisms underlying lineage segregation during mouse pre-implantation development using single-cell expression analysis. Currently I am a Daichii Sankyo RD Novare Co. Ltd. visiting scholar in the McManus lab and am excited to learn new things in a fantastic training environment. I am also interested in learning more about how to perform computational analysis of big data. I enjoy hiking, cycling, fishing and all the wonderful things the Bay Area offers.


recent publications

Ohnishi Y, Huber W, Tsumura A, Kang M, Xenopoulos P, Kurimoto K, et al. Cell-to-cell expression variability followed by signal reinforcement progressively segregates early mouse lineages.. Vol 16.; 2014. (Nat Cell Biol; vol 16; no 1).
Ohnishi Y, Totoki Y, Toyoda A, Watanabe T, Yamamoto Y, Tokunaga K, et al. Active role of small non-coding RNAs derived from SINE/B1 retrotransposon during early mouse development.. Vol 39.; 2012. (Mol Biol Rep; vol 39; no 2).
Lavial F, Bessonnard S, Ohnishi Y, Tsumura A, Chandrashekran A, Fenwick MA, et al. Bmi1 facilitates primitive endoderm formation by stabilizing Gata6 during early mouse development.. Vol 26.; 2012. (Genes Dev; vol 26; no 13).
Ohnishi Y, Totoki Y, Toyoda A, Watanabe T, Yamamoto Y, Tokunaga K, et al. Small RNA class transition from siRNA/piRNA to miRNA during pre-implantation mouse development.. Vol 38.; 2010. (Nucleic Acids Res; vol 38; no 15).
Tamura Y, Yoshida M, Ohnishi Y, Hohjoh H. Variation of gene silencing involving endogenous microRNA in mammalian cells.. Vol 36.; 2009. (Mol Biol Rep; vol 36; no 6).
Ohnishi Y, Tokunaga K, Kaneko K, Hohjoh H. Assessment of allele-specific gene silencing by RNA interference with mutant and wild-type reporter alleles.. Vol 2.; 2006. (J RNAi Gene Silencing; vol 2; no 1).