McManusLab dissecting the dark matter of our genome
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Renewable RNAi

  • Blau JA, McManus MT
    Nat Biotechnol.   |   April 5, 2013

A Systematic Mammalian Genetic Interaction Map Reveals Pathways Underlying Ricin Susceptibility

  • Michael C. Bassik, Martin Kampmann, Robert Jan Lebbink, Shuyi Wang, Marco Y. Hein, Ina Poser, Jimena Weibezahn, Max A. Horlbeck, Siyuan Chen, Matthias Mann, Anthony A. Hyman, Emily M. LeProust, Michael T. McManus, Jonathan S. Weissman
    Cell   |   February 7, 2013

T cell activation induces proteasomal degradation of Argonaute and rapid remodeling of the microRNA repertoire

  • Bronevetsky Y, Villarino AV, Eisley CJ, Barbeau R, Barczak AJ, Heinz GA, Kremmer E, Heissmeyer V, McManus MT, Erle DJ, Rao A, Ansel KM
    J Exp Med   |   February 4, 2013

IRE1α Cleaves Select microRNAs during ER Stress to Derepress Translation of Proapoptotic Caspase-2

  • Upton JP, Wang L, Han D, Wang ES, Huskey NE, Lim L, Truitt M, McManus MT, Ruggero D, Goga A, Papa FR, Oakes SA
    Science   |   October 4, 2012

RNA-Seq Reveals Unique Features of the Pancreatic β-Cell Transcriptome

  • Ku GM, Kim H, Vaughn IW, Hangauer MJ, Myung Oh C, German MS, McManus MT
    Mol Endocrinol.   |   October 1, 2012

Let-7b/c Enhance the Stability of a Tissue-Specific mRNA during Mammalian Organogenesis as Part of a Feedback Loop Involving KSRP

  • Emanuela Repetto, Paola Briata, Nathalie Kuziner, Brian D. Harfe, Michael T. McManus, Roberto Gherzi, Michael G. Rosenfeld, Michele Trabucchi
    PLoS Genetics   |   July 26, 2012

Right- and left-loop short shRNAs have distinct and unusual mechanisms of gene silencing.

  • Dallas A, Ilves H, Ge Q, Kumar P, Shorenstein J, Kazakov SA, Cuellar TL, McManus MT, Behlke MA, Johnston BH.
    NAR   |   July 18, 2012

Precursor MicroRNA-Programmed Silencing Complex Assembly Pathways in Mammals

  • Liu X, Jin DY, McManus MT, Mourelatos Z.
    Mol Cell.   |   April 11, 2012

A Resource for the Conditional Ablation of microRNAs in the Mouse

  • Chong Yon Park, Lukas T. Jeker, Karen Carver-Moore, Alyssia Oh, Huey Jiin Liu, Rachel Cameron, Hunter Richards, Zhongmei Li, David Adler, Yuko Yoshinaga, Maria Martinez, Michael Nefadov, Abul K. Abbas, Art Weiss, Lewis L. Lanier, Pieter J. de Jong, Jeffrey A. Bluestone, Deepak Srivastava, Michael T. McManus
    Cell Reports   |   March 29, 2012

Widespread RNA 3'-end oligouridylation in mammals

  • Choi YS, Patena W, Leavitt AD, McManus MT
    RNA   |   January 30, 2012

An siRNA Screen in Pancreatic Beta Cells Reveals a Role for Gpr27 in Insulin Production

  • Gregory M. Ku, Zachary Pappalardo, Chun Chieh Luo, Michael S. German, Michael T. McManus
    PLoS Genet   |   January 12, 2012

Polymerase II Promoter Strength Determines Efficacy of microRNA Adapted shRNAs

  • Robert Jan Lebbink, Maggie Lowe, Theresa Chan, Htet Khine, Xiaoyin Wang, Michael T. McManus
    PLoS ONE   |   October 21, 2011

Posttranscriptional silencing of effector cytokine mRNA underlies the anergic phenotype of self-reactive T cells.

  • Villarino AV, Katzman SD, Gallo E, Miller O, Jiang S, McManus MT, Abbas AK.
    Immunity   |   January 28, 2011

The microRNA-processing enzyme Dicer is dispensable for somite segmentation but essential for limb bud positioning.

  • Zhang Z, O'Rourke JR, McManus MT, Lewandoski M, Harfe BD, Sun X.
    Dev. Biol.   |   January 20, 2011

Analysis of microRNA knockouts in mice

  • Park CY, Choi YS, McManus MT.
    Hum Mol Genet.   |   September 15, 2010

Dicer1 and miR-219 Are Required for Normal Oligodendrocyte Differentiation and Myelination

  • Dugas JC, Cuellar TL, Scholze A, Ason B, Ibrahim A, Emery B, Zamanian JL, Foo LC, McManus MT, Barres BA.
    Neuron   |   March 11, 2010

Expanded RNA-binding activities of mammalian Argonaute 2.

  • Tan GS, Garchow BG, Liu X, Yeung J, Morris JP 4th, Cuellar TL, McManus MT, Kiriakidou M.
    Nucleic Acids Res. 37(22):7533-45.   |   December 1, 2009

Dicer ablation in oligodendrocytes provokes neuronal impairment in mice.

  • Shin D, Shin JY, McManus MT, Ptácek LJ, Fu YH
    Ann Neurol. 66(6):843-857   |   November 30, 2009

Lentivirus production

  • Wang X, McManus MT.
    J Vis Exp.   |   October 2, 2009

Dicer is required for proper liver zonation

  • Sekine S, Ogawa R, McManus MT, Kanai Y, Hebrok M.
    J. Pathol   |   July 27, 2009

Chd1 regulates open chromatin and pluripotency of embryonic stem cells

  • Gaspar-Maia A, Alajem A, Polesso F, Sridharan R, Mason MJ, Heidersbach A, Ramalho-Santos J, McManus MT, Plath K, Meshorer E, Ramalho-Santos M.
    Nature   |   July 8, 2009

Rapid creation and quantitative monitoring of high coverage shRNA libraries.

  • Bassik MC[1], Lebbink RJ[1], Churchman LS, Ingolia NT, Patena W, Leproust EM, Schuldiner M, Weissman JS, McManus MT.
    Nat Methods   |   May 17, 2009

LPS induces KH-type splicing regulatory protein-dependent processing of microRNA-155 precursors in macrophages.

  • Ruggiero, T., Trabucchi, M., De Santa, F., Zupo, S., Harfe, B. D., McManus, M. T., Rosenfeld, M. G., Briata, P., Gherzi, R.
    FASEB J.   |   May 7, 2009

Up-regulation of miR-21 by HER2/neu Signaling Promotes Cell Invasion.

  • Huang TH, Wu F, Loeb GB, Hsu R, Heidersbach A, Brincat A, Horiuchi D, Lebbink RJ, Mo YY, Goga A, McManus MT.
    J Biol Chem.   |   May 6, 2009

Conditional loss of Dicer disrupts cellular and tissue morphogenesis in the cortex and hippocampus

  • Davis TH, Cuellar TL, Koch SM, Barker AJ, Harfe BD, McManus MT, Ullian EM.
    J Neurosci.   |   April 23, 2009

Residual microRNA expression dictates the extent of inner ear development in conditional Dicer knockout mice

  • Soukup GA, Fritzsch B, Pierce ML, Weston MD, Jahan I, McManus MT, Harfe BD.
    Developmental Biology   |   April 15, 2009

Genetic analyses reveal a requirement for Dicer1 in the mouse urogenital tract

  • Pastorelli L, Wells S, Fray M, Smith A, Hough T, Harfe BD, McManus MT, Smith L, Woolf AS, Cheeseman M, Greenfield A
    Mammalian Genome   |   March 1, 2009

Sertoli cell Dicer is essential for spermatogenesis in mice

  • Papaioannou MD, Pitetti JL, Ro S, Park C, Aubry F, Schaad O, Vejnar CE, Kühne F, Descombes P, Zdobnov EM, McManus MT, Guillou F, Harfe BD, Yan W, Jégou B, Nef S.
    Dev Biol.   |   February 1, 2009

Podocyte-specific deletion of dicer alters cytoskeletal dynamics and causes glomerular disease

  • Harvey SJ, Jarad G, Cunningham J, Goldberg S, Schermer B, Harfe BD, McManus MT, Benzing T, Miner JH.
    J Am Soc Nephrol.   |   November 1, 2008

Mouse let-7 miRNA populations exhibit RNA editing that is constrained in the 5'-seed/ cleavage/anchor regions and stabilize predicted mmu-let-7a:mRNA duplexes

  • Reid JG, Nagaraja AK, Lynn FC, Drabek RB, Muzny DM, Shaw CA, Weiss MK, Naghavi AO, Khan M, Zhu H, Tennakoon J, Gunaratne GH, Corry DB, Miller J, McManus MT, German MS, Gibbs RA, Matzuk MM, Gunaratne PH.
    Genome Res.   |   October 1, 2008

Dicer1 is required for differentiation of the mouse male germline

  • Maatouk DM, Loveland KL, McManus MT, Moore K, Harfe BD.
    Biol Reprod.   |   October 1, 2008

Selective miRNA disruption in T reg cells leads to uncontrolled autoimmunity.

  • Zhou X, Jeker LT, Fife BT, Zhu S, Anderson MS, McManus MT, Bluestone JA.
    J Exp Med.   |   September 1, 2008

Dicer inactivation leads to progressive functional and structural degeneration of the mouse retina

  • Damiani D, Alexander JJ, O'Rourke JR, McManus M, Jadhav AP, Cepko CL, Hauswirth WW, Harfe BD, Strettoi E.
    J Neurosci.   |   May 7, 2008

Dicer loss in striatal neurons produces behavioral and neuroanatomical phenotypes in the absence of neurodegeneration

  • Cuellar TL, Davis TH, Nelson PT, Loeb GB, Harfe BD, Ullian E, McManus MT.
    Proc Natl Acad Sci U S A.   |   April 8, 2008

Unintentional miRNA ablation is a risk factor in gene knockout studies: a short report

  • Osokine I, Hsu R, Loeb GB, McManus MT.
    PLoS Genet.   |   February 1, 2008

Members of the miRNA-200 family regulate olfactory neurogenesis

  • Choi PS, Zakhary L, Choi WY, Caron S, Alvarez-Saavedra E, Miska EA, McManus M, Harfe B, Giraldez AJ, Horvitz HR, Schier AF, Dulac C.
    Neuron.   |   January 10, 2008

Behind the scenes of a small RNA gene-silencing pathway

  • Ku G, McManus MT.
    Hum Gene Ther.   |   January 1, 2008

MicroRNA expression is required for pancreatic islet cell genesis in the mouse

  • Lynn FC, Skewes-Cox P, Kosaka Y, McManus MT, Harfe BD, German MS.
    Diabetes.   |   December 1, 2007

Essential role for Dicer during skeletal muscle development

  • O'Rourke JR, Georges SA, Seay HR, Tapscott SJ, McManus MT, Goldhamer DJ, Swanson MS, Harfe BD.
    Dev Biol.   |   November 15, 2007

Extracellular sulfatases, elements of the Wnt signaling pathway, positively regulate growth and tumorigenicity of human pancreatic cancer cells

  • Nawroth R, van Zante A, Cervantes S, McManus M, Hebrok M, Rosen SD.
    PLoS ONE.   |   April 25, 2007

Dysregulation of cardiogenesis, cardiac conduction, and cell cycle in mice lacking miRNA-1-2

  • Zhao Y, Ransom JF, Li A, Vedantham V, von Drehle M, Muth AN, Tsuchihashi T, McManus MT, Schwartz RJ, Srivastava D.
    Cell.   |   April 20, 2007

Lentiviral Strategies for RNAi Knockdown of Neuronal Genes

  • Jill R. Crittenden1, Amy Heidersbach2, Michael T. McManus2
    Current Protocols in Neuroscience   |   April 15, 2007

Duox2 exhibits potent heme peroxidase activity in human respiratory tract epithelium

  • Harper RW, Xu C, McManus M, Heidersbach A, Eiserich JP.
    FEBS Lett.   |   October 2, 2006

RNA interference in embryonic stem cells and the prospects for future therapies

  • Heidersbach A, Gaspar-Maia A, McManus MT, Ramalho-Santos M.
    Gene Ther.   |   March 1, 2006

Dicer function is essential for lung epithelium morphogenesis

  • Harris KS, Zhang Z, McManus MT, Harfe BD, Sun X.
    Proc Natl Acad Sci U S A.   |   February 14, 2006

The microRNA miR-196 acts upstream of Hoxb8 and Shh in limb development

  • Hornstein E, Mansfield JH, Yekta S, Hu JK, Harfe BD, McManus MT, Baskerville S, Bartel DP, Tabin CJ.
    Nature.   |   December 1, 2005

MicroRNAs and endocrine biology

  • Cuellar TL, McManus MT.
    J Endocrinol.   |   December 1, 2005

Slowing down the Ras lane: miRNAs as tumor suppressors?

  • Morris JP 4th, McManus MT.
    Sci STKE.   |   August 16, 2005

TAZ, a transcriptional modulator of mesenchymal stem cell differentiation

  • Hong JH, Hwang ES, McManus MT, Amsterdam A, Tian Y, Kalmukova R, Mueller E, Benjamin T, Spiegelman BM, Sharp PA, Hopkins N, Yaffe MB.
    Science.   |   August 12, 2005

The RNaseIII enzyme Dicer is required for morphogenesis but not patterning of the vertebrate limb

  • Harfe BD, McManus MT, Mansfield JH, Hornstein E, Tabin CJ.
    Proc Natl Acad Sci U S A.   |   August 2, 2005

Small RNAs and immunity

  • McManus MT.
    Immunity.   |   December 1, 2004

MicroRNA-responsive 'sensor' transgenes uncover Hox-like and other developmentally regulated patterns of vertebrate microRNA expression

  • Mansfield JH, Harfe BD, Nissen R, Obenauer J, Srineel J, Chaudhuri A, Farzan-Kashani R, Zuker M, Pasquinelli AE, Ruvkun G, Sharp PA, Tabin CJ, McManus MT.
    Nat Genet.   |   October 1, 2004

Cre-lox-regulated conditional RNA interference from transgenes

  • Ventura A, Meissner A, Dillon CP, McManus M, Sharp PA, Van Parijs L, Jaenisch R, Jacks T.
    Proc Natl Acad Sci U S A.   |   July 13, 2004

MicroRNAs and cancer

  • McManus MT.
    Semin Cancer Biol.   |   August 1, 2003

RNA interference of influenza virus production by directly targeting mRNA for degradation and indirectly inhibiting all viral RNA transcription

  • Ge Q, McManus MT, Nguyen T, Shen CH, Sharp PA, Eisen HN, Chen J.
    Proc Natl Acad Sci U S A.   |   March 4, 2003

A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference

  • Rubinson DA, Dillon CP, Kwiatkowski AV, Sievers C, Yang L, Kopinja J, Rooney DL, Zhang M, Ihrig MM, McManus MT, Gertler FB, Scott ML, Van Parijs L.
    Nat Genet.   |   March 1, 2003

Small interfering RNA-mediated gene silencing in T lymphocytes

  • McManus MT, Haines BB, Dillon CP, Whitehurst CE, van Parijs L, Chen J, Sharp PA.
    J Immunol.   |   November 15, 2002

Gene silencing in mammals by small interfering RNAs

  • McManus MT, Sharp PA.
    Nat Rev Genet.   |   October 1, 2002

CP110, a cell cycle-dependent CDK substrate, regulates centrosome duplication in human cells

  • Chen Z, Indjeian VB, McManus M, Wang L, Dynlacht BD.
    Dev Cell.   |   September 1, 2002

Gene silencing using micro-RNA designed hairpins

  • McManus MT, Petersen CP, Haines BB, Chen J, Sharp PA.
    RNA   |   June 1, 2002

Identification of candidate mitochondrial RNA editing ligases from Trypanosoma brucei

  • McManus MT, Shimamura M, Grams J, Hajduk SL.
    RNA.   |   February 1, 2001

Processing of polycistronic guide RNAs is associated with RNA editing complexes in Trypanosoma brucei

  • Grams J, McManus MT, Hajduk SL.
    EMBO J.   |   October 16, 2000

Trypanosoma brucei guide RNA poly(U) tail formation is stabilized by cognate mRNA

  • McManus MT, Adler BK, Pollard VW, Hajduk SL.
    Mol Cell Biol.   |   February 1, 2000

Insertional and deletional RNA editing in trypanosome mitochondria

  • Hajduk SL, Adler B, Madison-Antenucci S, McManus M, Sabatini R.
    Nucleic Acids Symp Ser.   |   January 1, 1997