This is the protocol for site-directed mutagenesis based on the Stratagene kit.
Set up as follows:
Depending on the length of the plasmid, this program can become very long, so it may be best to run overnight.
Add 1uL of Dpn1 to PCR reaction. Incubate at 37 degrees Celsius for 1-2 hours to digest parental DNA. Run 5uL of the digested reaction on a gel and compare to the undigested parental plasmid - there should be some difference in band pattern. Transform into competent cells.