Input DNAs:Include phage promoter in the 5’-ends of your PCR products-orlinearizephage polymerase prooter vector (such as pBluescript).Make the ssRNA:Use mMessage mMachine kit [Ambion] to in vitro transcribe your DNAPurify the RNAs on a denaturing polyacrylamide gel, UV shadow, and elute in model buffer (seebelow). Precipitate and dissolve in H2O.Make the dsRNAssRNA and asRNA [0.5microM] in 10 mM Tris HCl [pH 7.5] with 20 mM NaCl heated at 95Cfor 1 minuteanneal at room temp for 12-16 hoursanalyze between 100-500 ng on a 2% agarose gel TBE buffer (stained with Ethidium Bromide