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CP110, a cell cycle-dependent CDK substrate, regulates centrosome duplication in human cells.
Publication Type:Journal Article
Source:Dev Cell, Volume 3, Issue 3, p.339-50 (2002)
Keywords:Amino Acid Motifs, Amino Acid Sequence, Cell Cycle Proteins, Centrosome, Cloning, Molecular, Cyclin-Dependent Kinases, DNA, Complementary, G1 Phase, Gene Expression Regulation, HeLa Cells, Humans, In Situ Hybridization, Fluorescence, Microtubule-Associated Proteins, Molecular Sequence Data, Mutagenesis, Site-Directed, Phosphoproteins, Phosphorylation, Polyploidy, Recombinant Proteins, RNA, Small Interfering, S Phase, Sensitivity and Specificity, Sequence Alignment, Substrate Specificity, Tumor Cells, Cultured
<p>Centrosome duplication and separation are linked inextricably to certain cell cycle events, in particular activation of cyclin-dependent kinases (CDKs). However, relatively few CDK targets driving these events have been uncovered. Here, we have performed a screen for CDK substrates and have isolated a target, CP110, which is phosphorylated by CDKs in vitro and in vivo. Human CP110 localizes to centrosomes. Its expression is strongly induced at the G1-to-S phase transition, coincident with the initiation of centrosome duplication. RNAi-mediated depletion of CP110 indicates that this protein plays an essential role in centrosome duplication. Long-term disruption of CP110 phosphorylation leads to unscheduled centrosome separation and overt polyploidy. Our data suggest that CP110 is a physiological centrosomal CDK target that promotes centrosome duplication, and its deregulation may contribute to genomic instability.</p>