Gene silencing in mammals by small interfering RNAs

Posted by lyu | Created: 15 Jun 2009 | Last Modified: 09 Jul 2009
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Authors: 
McManus MT, Sharp PA.
Journal: 
Nat Rev Genet.
Publication Date: 
October 1, 2002
Institutions: 
University of California, San Francisco
Abstract: 
Among the 3 billion base pairs of the human genome, there are approximately 30,000-40,000 protein-coding genes, but the function of at least half of them remains unknown. A new tool - short interfering RNAs (siRNAs) - has now been developed for systematically deciphering the functions and interactions of these thousands of genes. siRNAs are an intermediate of RNA interference, the process by which double-stranded RNA silences homologous genes. Although the use of siRNAs to silence genes in vertebrate cells was only reported a year ago, the emerging literature indicates that most vertebrate genes can be studied with this technology.
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