Active role of small non-coding RNAs derived from SINE/B1 retrotransposon during early mouse development.


Publication Type:

Source:

Mol Biol Rep, Volume 39, Issue 2, p.903-9 (2012)

Keywords:

Animals, Blastocyst, DEAD-box RNA Helicases, Electroporation, Embryonic Development, Gametogenesis, Gene Expression Regulation, Developmental, Gene Knockdown Techniques, High-Throughput Nucleotide Sequencing, Mice, Molecular Sequence Annotation, Oligonucleotides, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Ribonuclease III, RNA, Small Untranslated, Short Interspersed Nucleotide Elements

Abstract:

<p>Small RNAs derived from repetitive sequences appear to play essential roles in mammalian gametogenesis and early development. In this study we focused on the short interspersed nucleotide element B1 (SINE/B1) small RNAs, which were zygotically expressed in pre-implantation mouse embryos; and we investigated whether the SINE/B1 small RNAs played an active role in gene silencing during early mouse development. The results indicated that the level of silencing activity involving the SINE/B1 small RNAs as mediators was significantly reduced in Dicer-knockdown mouse embryos. In addition, when the SINE/B1 small RNAs were mapped to a full-length SINE/B1 sequence, phase-distribution of the small RNAs appeared, suggesting possible enzymatic involvement. Therefore, our present study suggested that the zygotically expressed SINE/B1 small RNAs in pre-implantation mouse embryos contain active small RNAs, which were presumably processed by Dicer and involved in gene silencing.</p>